Bone marrow stromal cell-conditioned medium regenerates injured sciatic nerve by increasing expression of MPZ and NGF and decreasing apoptosis.

Objectives: Despite the many benefits of mesenchymal stem cell (MSC) transplantation for tissue regeneration, there are some limitations to using them, including the high costs, applying invasive procedures, the possibility of transplant rejection, and cell malignancy. This study aimed to investigate the effect of secretions of bone marrow stromal cells (BMSCs) with the cell-free strategy on damaged sciatic nerve with an emphasis on the role of apoptosis and the expression of myelin protein zero (MPZ) and nerve growth factor (NGF) proteins. Materials and Methods: BMSCs were cultured and a 25-fold concentrated conditioned medium (CM) from the cells was provided. After creating a crush injury in the left sciatic nerve of male rats, BMSCs or CM were injected into the injured site of the nerve. Four weeks later, the expression of MPZ, NGF, Bax, and Bcl-2 proteins in the sciatic nerve and histological parameters of the sciatic nerve and gastrocnemius muscle were assessed. Results: The results demonstrated that injection of CM decreased apoptosis and increased expression of MPZ and NGF proteins, improving remyelination and regeneration of the sciatic nerve almost as much as the transplantation of the BMSCs themselves compared to the control group. Conclusion: The results suggest that BMSC secretions may improve remyelination and regeneration of damaged sciatic nerve by increasing the expression of MPZ and NGF and decreasing apoptosis.

Utilizing supply-demand bundles in Nature-based Recreation offers insights into specific strategies for sustainable tourism management.

Balancing supply and demand in Nature-based Recreation (NbR) has the potential to yield co-benefits across multiple Ecosystem Services (ES), helping to make tourism activities more sustainable. However, a comprehensive understanding of supply-demand mismatches in NbR is challenging due to the complex interaction among various social, economic and ecological factors. This paper investigates mismatches in NbR supply and demand to provide insights for informing spatial and regional planning to achieve sustainable tourism. To this end, the paper uses a wide range of indicators such as biophysical attributes, accessibility and social indicators to map and assess NbR supply and demand, followed by the application of spatial statistics to analyse supply-demand mismatches. Cluster analysis was performed based on the supply-demand relationship to identify a typology of NbR ES across the study area in the north of Iran. The paper proposes an innovative application of recreation ES bundles with potential implications for sustainable tourism in a region marked as a hot spot for tourism. The analysis generated a typology of five bundles of NbR ES with differing recreational opportunities. Bundles 1 and 2, characterized by a supply surplus and substantial ecological value, are suitable for NbR activities such as camping, hiking, climbing, and birdwatching. In contrast, bundle 4 and 5 associated with urban centres, experience a supply deficit, making them less suitable for NbR. Bundle 3, characterized by a mixture of natural and productive lands, plays an important role in maintaining a balanced supply-demand state. This region holds potential for diverse forms of tourism, including rural and agricultural recreation such as farm tours and farm life experiences. Based on findings, the paper provides valuable insights for spatial and regional planning by proposing targeted strategies to sustainably manage tourism activities.

Follicular Lymphoma Diagnosed With Medical Thoracoscopy.

Non-Hodgkin lymphomas may present with a recurrent pleural effusion, usually with involvement of other thoracic or extrathoracic sites. Lymphomas typically presenting with pleural disease include primary effusion lymphoma and pyothorax-associated lymphoma. We describe an unusual case of recurrent pleural effusion secondary to follicular lymphoma with no other known extrathoracic involvement at the time of diagnosis.

Human immune reactivity against liver sinusoidal endothelial cells from GalTα(1,3)GalT-deficient pigs.

Elimination of galactose-α(1,3)galactose (Gal) expression in pig organs has been previously shown to prevent hyperacute xenograft rejection. However, naturally present antibodies to non-Gal epitopes activate endothelial cells, leading to acute humoral xenograft rejection. Still, it is unknown whether xenogeneic pig liver sinusoidal endothelial cells (LSECs) from α(1,3)galactosyltransferase (GalT)-deficient pigs are damaged by antibody and complement-mediated mechanisms. The present study examined the xeno-antibody response of LSECs from GalT-deficient and wild pigs. Isolated LSEC from wild-type and GalT pigs were expose to human and baboon sera; IgM and IgG binding was analyzed by flow cytometry. Complement activation (C3a and CH50) was quantified in vitro from serum-exposed LSEC cultures using Enzyme-Linked ImmunoSorbent assay (ELISA). Levels of complement-activated cytotoxicity (CAC) were also determined by a fluorescent Live-Dead Assay and by the quantification of LDH release. IgM binding to GalT knockout (KO) LSECs was significantly lower (80% human and 87% baboon) compare to wild-type pig LSEC. IgG binding was low in all groups. Moreover, complement activation (C3a and CH50) levels released following exposure to human or baboon sera were importantly reduced (42% human and 52% baboon), CAC in GalT KO LSECs was reduced by 60% in human serum and by 72% in baboon serum when compared to wild-type LSECs, and LDH release levels were reduced by 37% and 57%, respectively. LSECs from GalT KO pigs exhibit a significant protection to humoral-induced cell damage compared to LSECs from wild pigs when exposed to human serum. Although insufficient to inhibit xenogeneic reactivity completely, transgenic GalT KO expression on pig livers might contribute to a successful application of clinical xenotransplantation in combination with other protective strategies.